Saturday, February 29, 2020

Biodegradation Using Fungal Isolates

Biodegradation Using Fungal Isolates Dr. Jayanthi Abraham* Anjali.M, Caroline Jessica Solomon, V.Vinothini. Abstract Biodegradation is a sustainable and eco friendly way to clean up contaminated environments. The biological processes harnessed play a major role in the removal of contaminants and they make use of the catabolic versatility of microorganisms to degrade/covert compounds which are toxic and difficult to breakdown. The study was done to determine the biodegradation of profenofos using fungal strains. Two fungal strains were isolated and characterized by 18srRNA sequencing. Minimum inhibitory concentration was checked on the fungal isolates. Growth kinetics was performed using Czpax Dox broth to understand the growth pattern of the fungal isolates in the presence and absence of profenofos. The biodegradation of the pesticide was then performed using the isolates grown on minimal and mineral media. Analytical characterizations of profenofos were also done using HPLC, GCMS, and FTIR. Key words: Profenofos, Gro wth kinetics, Biodegradation, fungal isolates. 1. Introduction Modern agricultural practices include the use of various chemical supplements in the form of pesticides such as fungicides, herbicides, and insecticides which are used to manage different groups of pests to maximize crop yield and to meet the demands for higher supplies of food and other agricultural products for the fast-growing human population. Various  benefits are derived from the use of pesticides, however they also cause a wide array of toxicological and environmental problems and often persist in the environment for long periods of time. Organophosphorus insecticides are one of the most commonly used pesticides in agricultural practices. Out of a large number of organophosphate pesticides, profenofos is the one that is most widely used because of its broad spectrum of activity. Profenofos: O-4-bromo-2-chlorophenyl-O-ethyl S-propyl phosphorothioate (C11H15BrClO3PS, Molecular mass 373.6 g/mol]) is widely used for agricultural purposes in India and some other countries such as Australia, China, Pakistan, Egypt and Korea. [1][2][3][4][5][6].It is classified as moderately hazardous(toxicity class II) pesticide by WHO. Profenofos iw widely used on cotton, to control the tobacco budworm, cotton bollworm, armyworm, cotton aphid, mealy bugs, cotton strainers, whiteflies, spider mites, plant bugs, leafhoppers and flea hoppers ,they are used as effective pesticide for watermelon, Chilli peppers, tomatoes, soya beans . An estimated 85% of all profenofos is used to control lepidopteron species (the worm complex) at varying rates. It is a non systemic insecticide and acaricide which is found to have contact and stomach action. Like any other pesticide its effect on non target organisms is unavoidable and they eventually find their way into the food chain. Morphological, reproductive, enzymatic, hormonal and genotoxic effects exhibited by this pesticide have been reported[2]. Profenofos was found to be highly toxic to different organisms including mammals [8] insects  [9], and [6][11] Profenofos has been found to cause cholinesterase inhibition in humans; it over stimulates the nervous system causing nausea, dizziness, confusion, and at very high exposures, respiratory paralysis and death. It has been found that various bacteria such as Pseudomonas diminuta, Flavobacteria sp , Pseudomonas putida , Burkholderia gladioli [12] Pesudomonas aeruginosa , Staphylococcus aureus and Bacillus subtilis . fungi and actinomycetes [13] are able to degrade these pesticides in the soil. Fungi however have been found to be more efficient in doing so. There are several methods which can be used to degrade pesticides which have accumulated in the environment, these process are Photo catalytic degradation, Ozonation, Oxidative processes, Phytoremediation, photo-fenton reactions and bioremediations (Zenilda et al.). Of all these systems bioremediations is found to be most effective because it brings about complete mineralisation unlike in the case of physicalor chemical treatment. Treatment can be done insitu without having to excavate contaminated soil, thus it becomes extremely cost effective.

Thursday, February 13, 2020

Wounding With Intent and Malicious Infliction of Grievous Bodily Harm Essay

Wounding With Intent and Malicious Infliction of Grievous Bodily Harm or Wounding - Essay Example This paper illustrates that because Stan did not intend to cause grievous bodily harm but only intended to instill fear in Helen, his mental state is insufficient to establish the mens rea requirement for wounding with intent. Malice, however, which is the required state of mind for malicious infliction of grievous bodily harm or wounding, can be inferred by the defendant’s conduct and is unrelated to his motive, according to the leading case of Re A (children) (conjoined twins) [2000] 4 All ER 961. While Stan claims that he did not intend to inflict bodily injuries, and his motive was to make her afraid rather than to hurt her, general malice can be inferred by his act of throwing a bottle directly at Helen. Thus, the mens rea requirement for the lesser offenses of malicious infliction of grievous bodily harm or malicious wounding is all that can be established from the facts. Depending on whether or not Helen’s injuries were a wound or were grievous under the Offences Against The Person Act of 1861 s.20, Stan may have committed a malicious infliction of grievous bodily harm or malicious wounding. The Act prohibits the unlawful and malicious wounding of another person (meaning the â€Å"breaking of the continuity of the whole of the outer skin, or the inner skin within the cheek or lip†) as well as the unlawful and malicious infliction of grievous, or serious, bodily harm (a jury question). If both a wound and grievous bodily harm exist, R v McCready [1978] 1 WLR 1376 mandates that the correct charge is unlawful wounding. The bottle that Stan threw at Helen caused a deep cut that required stitches. Thus the breaking of the skin requirement for unlawful wounding is clearly met. Grievous bodily harm may also be present since such a deep cut is arguably serious according to the plain meaning of the word, but this question does not need to be addressed since the holding in McCready requires that the charge be unlawful wounding under s.20 if a wound is present. Thus, the crime that Stan probably committed is malicious wounding.

Saturday, February 1, 2020

NBC Internet TV Assignment Example | Topics and Well Written Essays - 1000 words

NBC Internet TV - Assignment Example The Federal Communications Commission (FCC) has blocked active online streaming sites with temporary restraining orders, saying permission is not granted to broadcast without paying copyright fees. Some companies agree that the FCC needs to â€Å"clearly define what an online video distributor is† and post clear and concise rules that consider all stakeholders for this new type of broadcasting across the Internet (Kang, 2010). In order for NBC to be a competitive force in the Internet TV arena, they must offer connection to all forms of Internet technology that is being used regularly. NBC is interested in continuing to broadcast news, shows, and other programs on the Internet TV, claiming the ability to reach a much larger audience than regular TV. Opposition to NBC’s close-circuit broadcasts believe that anyone who owns a television capable of connecting to the Internet should have access to everything the Web has to offer (Kang, 2010). Reports that detail the network s that currently block viewing privileges of web-based programs to some Internet TV subscribers has some interested parties upset. The idea that big conglomerates can oust smaller organizations before they even have a chance to be successful is unfair monopolization of the industry, according to business attorneys for the opposition (Kang, 2010). The ability to reach a larger audience by broadcasting across Internet TV allows NBC to increase revenue through increased ad sales and other vendor payments that would otherwise be invalid, were it not for global inclusion. The threat of having to share content provided by NBC to other Internet TV users could hamper the revenue collected by NBC. Companies and trade firms involved in Internet TV would like to see FCC rules and regulation maintain â€Å"the integrity of the pay stream† that has been established (Kang, 2010). The Nielson ratings and demographic coverage supply the data used by television stations to sell future adverti sements. Tapping into the Internet TV market allows data statistics to increase, thus revenue to potentially increase for NBC (Robertson, 2012). The success or failure of putting NBC on Internet TV may very likely be attributed to the conditions the FCC puts on this and other television stations wanting to broadcast across the globe. The ability for NBC to compete in the Internet TV arena will include their willingness to do what it takes to maintain the seamless Internet connectivity desired by those loyal customers who will continue to support NBC. Rogers Internet TV Rogers On Demand Online, one of the three largest broadcasting networks in Canada, changed their name to Rogers Anyplace TV (RogersKaili, 2012) to incorporate the idea that viewing of programming can happen anywhere or anytime. Rogers has opened the access of Internet TV in Canada to personal computers, smartphones, tablets, and the Xbox360 (Suppa, 2010) giving viewers more options of scheduling and availability of pr ograms. The Internet TV options in Canada are expanding due to Rogers efforts to open up alternative ways for customers to continuously stream programs into busy schedules. The offering of â€Å"play-shifting, time-shifting, micro-transactional, and high definition content delivery† (Suppa, 2010) have made Rogers the first company to expand this extensively into Internet TV. The idea behind the name is that the more options a customer has to